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  • Title: [Optimization on ultrasonic extraction technology of total coumarines in cortex fraxini].
    Author: Xiong LZ, Ma Q, Long H.
    Journal: Zhong Yao Cai; 2012 Apr; 35(4):634-7. PubMed ID: 23019911.
    Abstract:
    OBJECTIVE: To optimize the ultrasonic extraction technology of total coumarins in Cortex Fraxini. METHODS: The effects of ethanol concentration, ratio of solid to liquid, extraction temperature, ultrasonic frequency and extraction time in extraction procedure on the extraction rate of total coumarins in Cortex Fraxini was researched by single factor experiment, the optimal extraction technology was determined by orthogonal experiment. RESULTS: The optimal extraction technology of total coumarins in Cortex Fraxini was:mixing the material with 60% ethanol according to ratio of solid to liquid 1:10 (W/V), ultrasonic frequency 175 W and extracting 40 min at 50 degrees C. The extraction rate of total coumarins reached 6.283% with the optimal extraction technology. CONCLUSION: Compares with the classic Soxhlet extraction, ultrasound is a less time-comsuming, low energy and great efficiency tool for the fast extraction of coumarins from Cortex Fraxini.
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