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  • Title: In-situ synthesis of fluorescent gold nanoclusters with electrospun fibrous membrane and application on Hg (II) sensing.
    Author: Cai Y, Yan L, Liu G, Yuan H, Xiao D.
    Journal: Biosens Bioelectron; 2013 Mar 15; 41():875-9. PubMed ID: 23021839.
    Abstract:
    In the present study, a method for in-situ preparation of fluorescent gold nanoclusters with bovine serum albumin/poly(ethylene oxide) electrospun membrane (BSA/PEO-Au NCs) has been developed to detect Hg(2+). In this work, Au NCs were immobilized both inside and on the surface of fibrous membrane using one-step reduction of HAuCl(4) by BSA. The as-prepared BSA/PEO-Au NCs were able to emit intensive red fluorescence under the excitation of visible light, and the fluorescence could be quenched by Hg(2+). The results indicate that the as-prepared BSA/PEO-Au NCs exhibit good sensitivity towards Hg(2+), which might be benefit from the large specific surface area of BSA/PEO fibrous membrane. The biosensor exhibits a linear response to Hg(2+) concentrations ranging from 0.5 nM to 75 nM with a limit of detection (LOD) of 57 pM. Moreover, BSA/PEO-Au NCs shows good selectivity towards other common metal ions. With the advantages of the simple preparation process and good detection performance, the proposed method is anticipated to fabricate other sensitive fluorescence fiber-based sensors of metal nanoclusters.
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