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  • Title: A comparison of results for cholesterol in human serum obtained by the Reference Method and by the Definitive Method of the National Reference System for cholesterol.
    Author: Ellerbe P, Myers GL, Cooper GR, Hertz HS, Sniegoski LT, Welch MJ, White E.
    Journal: Clin Chem; 1990 Feb; 36(2):370-5. PubMed ID: 2302783.
    Abstract:
    Here we compare the Reference Method (I) and the Definitive Method (II) for measurement of cholesterol in serum. For pure cholesterol solutions, values by I agree with values by II, but for fresh, frozen, or lyophilized sera, values by I average 1.6% higher than values by II. We conclude that an undetected interference is associated with I. However, the observed difference does not diminish the usefulness of I as an accuracy base for cholesterol measurements, because it is clinically insignificant at present.
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