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  • Title: A method for fast analysis of volatile components of Citrus aurantium L. leaves.
    Author: Gholivand MB, Piryaei M.
    Journal: Nat Prod Res; 2013; 27(14):1315-8. PubMed ID: 23039826.
    Abstract:
    To avoid the traditional and time consuming hydrodistillation, the analyses of volatile components in leaves of Citrus aurantium L. were carried out by a simple microwave distillation followed by headspace single drop microextraction (MD-HS-SDME) coupled to gas chromatography-mass spectrometry (GC-MS). The headspace volatile compounds were collected after irradiation using a single drop of n-heptadecan. The extraction conditions were optimized using the relative peak areas as index. The chemical composition of the MD-HS-SDME extracts was confirmed according to their retention indexes and mass spectra. Forty-one components were extracted and identified by using the MD-HS-SDME method. Limonene (62%), Linalool (7.47%), trans-β-Ocimene (3.47%), Caryophyllene (2.05%), α- Bisabolene (1.8%) and Myrcene (1.18%) were the major constituents.
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