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  • Title: Biochemical and genetic analysis of NAD(P)H:quinone oxidoreductase 1 (NQO1).
    Author: Siegel D, Kepa JK, Ross D.
    Journal: Curr Protoc Toxicol; 2007; Chapter 4():Unit4.22. PubMed ID: 23045144.
    Abstract:
    NAD(P)H:quinone oxidoreductase 1 (NQO1, DT-diaphorase, E.C. 1.6.99.2) is an FAD containing obligate two-electron reductase that catalyzes the reduction of a broad range of substrates. This unit will describe methods for the detection of NQO1 protein in formalin-fixed, paraffin-embedded tissues by immunohistochemistry; detection of NQO1 protein in fresh tissues or cell lines by immunoblot analysis; measurement of NQO1 catalytic activity in fresh and frozen tissues and cell lines using spectrophotometric assays based upon the reduction of 2,6-dichlorophenol-indophenol (DCPIP) or coupled menadione-cytochrome reduction; and determination of the NQO1*2 polymorphism by PCR-RFLP.
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