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  • Title: [Effect of decorin gene transfection on human gastric carcinoma xenograft growth in nude mice].
    Author: Liu B, Jiang Z, Wang C, Hu ZM, Ji W, Cai C, Kang YJ.
    Journal: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi; 2012 Oct; 28(10):1009-12. PubMed ID: 23046927.
    Abstract:
    AIM: To investigate the effect of decorin (DCN) gene transfection on the growth of nude mice xenograft bearing human gastric carcinoma cell SGC-7901 and study the possible mechanism. METHODS: Gastric carcinoma xenograft model was established in nude mice. Recombinant plasmid pEGFP-N1-DCN was injected to the center and bottom of the carcinoma; meanwhile, the empty plasmid or physiological saline was injected in the other models as controls. We monitored the changes of carcinoma volume and weight, detected the expression of DCN by RT-PCR and Western blotting, the expressions of transforming growth factor-β1 (TGF-β1) and matrix metalloproteinases-9 (MMP-9) by Western blotting, and the expression of vascular endothelial growth factor (VEGF) by immunohistochemistry. RESULTS: Gastric carcinoma xenograft models were constructed successfully in nude mice. Compared with the control groups, slower growth speed, smaller volume and lighter weight were found in recombinant plasmid group (P<0.05). RT-PCR showed that the expression of DCN gene in recombinant plasmid group was significantly higher than that in the control groups (P<0.05); Western blotting indicated the expression of fusion protein in recombinant plasmid group; Western blotting also showed that the expressions of TGF-β1 and MMP-9 protein were lower in recombinant plasmid group than in the control groups (P<0.05); immunohistochemistry revealed that the expression of VEGF was down-regulated in recombinant plasmid group (P<0.05). CONCLUSION: DCN gene can inhibit the growth of nude mice xenograft, which is probably related with the decreased expressions of TGF-β1 and MMP-9 protein and the inhibition of tumor angiogenesis.
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