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  • Title: Progress-curve equations for reversible enzyme-catalysed reactions inhibited by tight-binding inhibitors.
    Author: Szedlacsek SE, Ostafe V, Duggleby RG, Serban M, Vlad MO.
    Journal: Biochem J; 1990 Feb 01; 265(3):647-53. PubMed ID: 2306205.
    Abstract:
    The rate equation for a tight-binding inhibitor of an enzyme-catalysed first-order reversible reaction was used to derive two integrated equations. One of them covers the situations in which competitive, uncompetitive or non-competitive inhibition occurs and the other refers to the special non-competitive case where the two inhibition constants are equal. For these equations, graphical and non-linear regression methods are proposed for distinguishing between types of inhibition and for calculating inhibition constants from progress-curve data. The application of the non-linear regression to the analysis of stimulated progress curves in the presence of a tight-binding inhibitor is also presented. The results obtained are valid for any type of 'dead-end'-complex-forming inhibitor and can be used to characterize an unknown inhibitor on the basis of progress curves.
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