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  • Title: Construction of ratiometric fluorescent sensors by ribonucleopeptides.
    Author: Annoni C, Nakata E, Tamura T, Liew FF, Nakano S, Gelmi ML, Morii T.
    Journal: Org Biomol Chem; 2012 Nov 28; 10(44):8767-9. PubMed ID: 23069733.
    Abstract:
    Ratiometric fluorescent sensors were constructed from RNA aptamers by generating modular ribonucleopeptide complexes. Fluorescent ribonucleopeptides containing fluorophore seminaphthorhodafluor tethered to their peptide subunit revealed a dual emission property, which permitted a ratiometric fluorescent measurement of a substrate-binding event. The strategy successfully afforded ratiometric fluorescent sensors for biologically active small ligands, tetracycline, dopamine and streptomycin.
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