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  • Title: [Use of a polarographic method for determining trichothecin].
    Author: Krugliak EB, Bilushi SG, Zaĭtsev PM, Korshunova LG.
    Journal: Antibiotiki; 1977 Dec; 22(12):1088-93. PubMed ID: 23070.
    Abstract:
    The polarographic behaviour of trichothecin was studied. It was shown that the antibiotic could be detected in solutions at concentrations of 7.10(-7) moles with the help of the polarographic method. Conditions for the polarographic determination of trichothecin in fermentation broth were developed. The error was not more than 3 per cent. The reliability of the results was shown by statistical treatment of data performed in accordance with the requirement of the USSR State Pharmacopeia, X ed., prescribing that the precision of the assay is such that the fiducial limits at p = 95 per cent deviate from the average value by not more than 5 per cent. Comparison of the results of trichothecin determination in the fermentation broth with the polarographic and biological methods showed no significant difference. Therefore, the polarographic method may be recommended for trichothecin determination in the fermentation broth.
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