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  • Title: 2-Deoxy-D-glucose uptake in cultured human muscle cells.
    Author: Jacobs AE, Oosterhof A, Veerkamp JH.
    Journal: Biochim Biophys Acta; 1990 Mar 09; 1051(3):230-6. PubMed ID: 2310773.
    Abstract:
    Hexose uptake was studied with cultured human muscle cells using 2-deoxy-D-[1-3H]glucose. At a concentration of 0.25 and 4 mM, phosphorylation rather than transport was the rate-limiting step in the uptake of 2-deoxy-D-glucose. This was not due to inhibition of the hexokinase activity by either ATP depletion or 2-deoxyglucose 6-phosphate accumulation. In cellular homogenates, hexokinase showed a lower Km value for glucose as compared to 2-deoxyglucose. Intact cells preferentially phosphorylated glucose instead of 2-deoxyglucose. Therefore, transport instead of phosphorylation may be rate limiting in the uptake of glucose by cultured human muscle cells. These data suggest caution in using 2-deoxyglucose for measuring glucose transport.
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