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  • Title: Parameters involved in the in vitro immunization of tonsilar lymphocytes: effects of rIL-2 and muramyl dipeptide.
    Author: Carroll K, Prosser E, O'Kennedy R.
    Journal: Hybridoma; 1990 Feb; 9(1):81-9. PubMed ID: 2312133.
    Abstract:
    A method for the in vitro immunization of human tonsilar lymphocytes is described. This method allows for the sensitization of B-cells against various antigens under serum-free medium conditions. The medium is supplemented with defined concentrations of antigen, rIL-2 and muramyl dipeptide to boost the immune responses. This method may prove useful for the generation of antigen reactive B-cells for use in human monoclonal antibody production and the study of the effects of other lymphokines on the in vitro immune response.
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