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  • Title: Gateway vectors for transformation of cereals.
    Author: Karimi M, Inzé D, Van Lijsebettens M, Hilson P.
    Journal: Trends Plant Sci; 2013 Jan; 18(1):1-4. PubMed ID: 23121806.
    Abstract:
    Until now, the availability of vectors for transgenic research in cereal crops has been rather limited. We present a novel collection of Agrobacterium tumefaciens binary T-DNA vectors compatible with Gateway recombinational cloning that facilitate the modular assembly of genes of interest together with new regulatory sequences, such as strong constitutive or endosperm-specific Brachypodium distachyon promoters. This resource aims at streamlining the creation of vectors and transgenes designed to explore gene functions in vital monocotyledonous crops.
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