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  • Title: [The effect of biyuanshu oral liquid on the formation of Pseudomonas aeruginosa biofilms in vitro].
    Author: Liu X, Chen H, Wang S.
    Journal: Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi; 2012 Jul; 26(14):640-3. PubMed ID: 23156807.
    Abstract:
    OBJECTIVE: To observe the effect of biyuanshu oral liquid on the formation of pseudomonas aeruginosa biofilms in vitro. METHOD: Pseudomonas aeruginosa biofilm was established by plate culture and detected by Scanning electron microscopy and AgNO3 staining. After treated with different dosages of biyuanshu oral liquid and erythromycin, the pseudomonas aeruginosa biofilms were observed by AgNO3 staining and the number of viable bacteria were measured by serial dilution. RESULT: The pseudomonas aeruginosa biofilms could be detected by SEM at the seventh culture day and it was consistent with the detection of AgNO3 staining. The biyuanshu oral liquid and erythromycin have the effect on inhibiting the formation of pseudomonas aeruginosa biofilms. But with the already formed pseudomonas aeruginosa biofilms the inhibition was not significant. The serial dilution method showed that the viable counts of bacteria of biyuanshu oral liquid and erythromycin treated groups were significantly lower than those untreated groups (P < 0.05). CONCLUSION: The biyuanshu oral liquid and erythromycin can inhibit the formation of pseudomonas aeruginosa biofilms in vitro.
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