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  • Title: Use of metaphase I oocytes matured in vitro is associated with embryo multinucleation.
    Author: De Vincentiis S, De Martino E, Buffone MG, Brugo-Olmedo S.
    Journal: Fertil Steril; 2013 Feb; 99(2):414-21. PubMed ID: 23158932.
    Abstract:
    OBJECTIVE: To evaluate the impact of oocyte maturational stage at retrieval on embryo multinucleation. DESIGN: Retrospective study. SETTING: Private institution for assisted reproduction. PATIENT(S): A total of 412 patients undergoing 500 intracytoplasmic sperm injection (ICSI) cycles between August 2006 and September 2010. INTERVENTION(S): Routine ICSI laboratory procedures. MAIN OUTCOME MEASURE(S): Normal and abnormal fertilization; embryo development; arrest at pronuclear stage; failure to undergo first mitotic division; presence of embryo multinucleation; embryo quality; pregnancy, implantation, and miscarriage rates. RESULT(S): A significantly lower percentage of multinucleation was found in embryos originating from metaphase II (MII) oocytes when compared with MI-II- and MI-derived oocytes. Significantly fewer multinucleated cells per embryo were observed in MII-derived oocytes. Clinical pregnancy and implantation rates were significantly higher when only embryos derived from MII oocytes were transferred. CONCLUSION(S): Embryo multinucleation rate increases when in vitro-matured (2-5 hours incubation) MI (MI-II) oocytes are used instead of in vivo-matured oocytes in ICSI. Furthermore, all other ICSI outcome parameters are also compromised. The use of donated gametes does not modify these results.
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