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  • Title: Temperature- and salinity-decoupled overproduction of hydroxyectoine by Chromohalobacter salexigens.
    Author: Rodríguez-Moya J, Argandoña M, Iglesias-Guerra F, Nieto JJ, Vargas C.
    Journal: Appl Environ Microbiol; 2013 Feb; 79(3):1018-23. PubMed ID: 23160137.
    Abstract:
    Hydroxyectoine overproduction by the natural producer Chromohalobacter salexigens is presented in this study. Genetically engineered strains were constructed that at low salinity coexpressed, in a vector derived from a native plasmid, the ectoine (ectABC) and hydroxyectoine (ectD) genes under the control of the ectA promoter, in a temperature-independent manner. Hydroxyectoine production was further improved by increasing the copies of ectD and using a C. salexigens genetic background unable to synthesize ectoines.
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