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  • Title: A novel amperometric immunosensor constructed with gold-platinum nanoparticles and horseradish peroxidase nanoparticles as well as nickel hexacyanoferrates nanoparticles.
    Author: Zhu Q, Yuan R, Chai Y, Han J, Li Y, Liao N.
    Journal: Analyst; 2013 Jan 21; 138(2):620-6. PubMed ID: 23172041.
    Abstract:
    In this study, three nano-materials comprising gold-platinum nanoparticles (Au-PtNPs), horseradish peroxidase nanoparticles (HRPNPs) and nickel hexacyanoferrate nanoparticles (NiHCFNPs) were used to construct a signal-off immunosensor. Au-PtNPs and NiHCFNPs were assembled on a glass carbon electrode (GCE) by electrodeposition and gold-cyanide bond formation, respectively; anti-fetoproteins (anti-AFP) were immobilized on the Au-PtNPs. HRPNPs were employed to block the possible remaining active sites and avoid nonspecific adsorption. Here, NiHCFNPs served as redox probes, while Au-PtNPs and HRPNPs were used for the synergistic catalysis of H(2)O(2) to amplify the signal. With more and more immunocomplex produced by the antibody-antigen reaction covering the biosensing surface, thus hindering electron transfer, the catalytic peak current will decrease quantitatively in relation to the concentration of target antigen. The resulting immunosensors exhibited a fast response and excellent sensitivity to α-fetoprotein (AFP), and showed two linear ranges in the concentration ranges of 0.06-13 ng mL(-1) and 13-200 ng mL(-1) with a detection limit of 0.017 ng mL(-1).
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