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Title: Isolation and characterization of hyaluronidase from scorpion (Heterometrus fulvipes) venom. Author: Ramanaiah M, Parthasarathy PR, Venkaiah B. Journal: Biochem Int; 1990; 20(2):301-10. PubMed ID: 2317213. Abstract: Hyaluronidase (Hyaluronate lyase, E.C. 3.2.1.35) has been isolated from Heterometrus fulvipes scorpion venom by a combination of gel filtration on Sephadex G-75 and ion exchange chromatography on DEAE-cellulose. The enzyme preparation showed a single band on polyacrylamide gel electrophoresis and a molecular weight of 82,000. The final preparation was purified 27-fold. The optimum pH for enzyme activity was 4.0. No loss of activity was observed up to 30 degrees C and showed a sharp decrease in activity at 50 degrees C. Heparin inhibited the enzyme activity.[Abstract] [Full Text] [Related] [New Search]