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  • Title: Aptasensors for rapid detection of Escherichia coli O157:H7 and Salmonella typhimurium.
    Author: Wu WH, Li M, Wang Y, Ouyang HX, Wang L, Li CX, Cao YC, Meng QH, Lu JX.
    Journal: Nanoscale Res Lett; 2012 Nov 29; 7(1):658. PubMed ID: 23190695.
    Abstract:
    Herein we reported the development of aptamer-based biosensors (aptasensors) based on label-free aptamers and gold nanoparticles (AuNPs) for detection of Escherichia coli (E. coli) O157:H7 and Salmonella typhimurium. Target bacteria binding aptamers are adsorbed on the surface of unmodified AuNPs to capture target bacteria, and the detection was accomplished by target bacteria-induced aggregation of the aptasensor which is associated as red-to-purple color change upon high-salt conditions. By employing anti-E. coli O157:H7 aptamer and anti-S. typhimurium aptamer, we developed a convenient and rapid approach that could selectively detect bacteria without specialized instrumentation and pretreatment steps such as cell lysis. The aptasensor could detect as low as 105colony-forming units (CFU)/ml target bacteria within 20 min or less and its specificity was 100%. This novel method has a great potential application in rapid detection of bacteria in the near future.
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