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  • Title: Microsatellite loci for tucumã of Amazonas (Astrocaryum aculeatum) and amplification in other Arecaceae.
    Author: Ramos SL, de Macêdo JL, Lopes MT, Batista JS, Formiga KM, da Silva PP, Saulo-Machado AC, Veasey EA.
    Journal: Am J Bot; 2012 Dec; 99(12):e508-10. PubMed ID: 23211567.
    Abstract:
    PREMISE OF THE STUDY: Microsatellite loci were developed for tucumã of Amazonas (Astrocaryum aculeatum), and cross-species amplification was performed in six other Arecaceae, to investigate genetic diversity and population structure and to provide support for natural populations management. • METHODS AND RESULTS: Fourteen microsatellite loci were isolated from a microsatellite-enriched genomic library and used to characterize two wild populations of tucumã of Amazonas (Manaus and Manicoré cities). The investigated loci displayed high polymorphism for both A. aculeatum populations, with a mean observed heterozygosity of 0.498. Amplification rates ranging from 50% to 93% were found for four Astrocaryum species and two additional species of Arecaceae. • CONCLUSIONS: The information derived from the microsatellite markers developed here provides significant gains in conserved allelic richness and supports the implementation of several molecular breeding strategies for the Amazonian tucumã.
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