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  • Title: [Study on HPLC fingerprints of Chrysanthemi Flos before and after sulfur-fumigation].
    Author: Liu X, Cai H, Ma XQ, Pei K, Cai BC.
    Journal: Zhong Yao Cai; 2012 May; 35(5):705-8. PubMed ID: 23213731.
    Abstract:
    OBJECTIVE: To evaluate the quality of Chrysanthemi Flos before and after sulfur-fumigation by analysis of HPLC fingerprints. METHODS: RP-HPLC method was applied to establish the chromatographic fingerprint. The separation was performed on a Waters C, (250 mm x 4.6 mm, 5 microm) column with a gradient elution composed of acetonitrile-aqueous solution containing 0.5% phosphoric acid. The column temperature was 35 degrees C, the detective wavelength was set at 268 nm. RESULTS: Eleven batches of Chrysanthemi Flos samples composed of both sun-dried and sulfur-fumigated were analyzed and evaluated respectively. The method of clustering analysis was used, and the chromatographic fingerprint common pattern was finally established. CONCLUSION: The HPLC fingerprint method is reliable and suitable for quality evaluation of Chrysanthemi Flos before and after sulfur-fumigation.
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