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  • Title: [Study on the HPLC-DAD fingerprint chromatograms of Radix Rubiae from different areas in China].
    Author: Yuan X, Gao JF, Yuan P.
    Journal: Zhong Yao Cai; 2012 May; 35(5):709-11. PubMed ID: 23213732.
    Abstract:
    OBJECTIVE: To establish the HPLC-DAD fingerprint of Radix Rubiae and provide a quality control method for it. METHODS: The method HPLC-DAD was performed on a YMC C,18 (250 mm x 4.6 mm, 5 microm) column with gradient elution of acetonitrile-0.2% phosphoric acid solution as mobile phase, flow rate at 1.0 mL/min, detection wavelength at 280 mm, and the column temperature at 25 degrees C, and the analysis time was 60 min. RESULTS: The average similarity of the fnigerprint chromatograms was over 0.94 in 6 batches samples of different areas in China. CONCLUSION: This method can be applied for the determination of Radix Rubiae from different areas.
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