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  • Title: Isolation of the retinal isomers from the isomerization of all-trans-retinal by flash countercurrent chromatography.
    Author: He M, Du W, Du Q, Zhang Y, Li B, Ke C, Ye Y, Du Q.
    Journal: J Chromatogr A; 2013 Jan 04; 1271(1):67-70. PubMed ID: 23219476.
    Abstract:
    The isolation of the retinal isomers from all-trans-retinal was performed by flash countercurrent chromatography. In each separation, isomerization reaction solution of 200mg all-trans-retinal could be loaded on a 1200 mL of high-speed countercurrent chromatographic column with 5mm bore, eluted by a mobile phase flow rate of 25 mL/min, resulting in 63 mg of 11-cis-retinal, 24 mg of 13-cis-retinal and 26 mg of 9-cis-retinal with purities more than 95%. n-Hexane-acetonitrile (3:1) was used as the solvent system which possesses the advantages of simplicity, re-use of the solvent and multiple injections. This method could be used to prepare 13-cis-retinal, 11-cis-retinal and 9-cis-retinal for the photoisomerization investigation, such as the effect of 11-cis-retinal in the visual system.
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