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  • Title: Monoclonal antibodies against human synovial phospholipase A2.
    Author: Takayama K, Kudo I, Hara S, Murakami M, Matsuta K, Miyamoto T, Inoue K.
    Journal: Biochem Biophys Res Commun; 1990 Mar 30; 167(3):1309-15. PubMed ID: 2322277.
    Abstract:
    Four monoclonal antibodies (HP-1, HP-2, HP-3 and HP-4) with differing reactivities were raised against human synovial fluid phospholipase A2. None of them bound to exocrine phospholipases A2, such as those from pancreas or snake venom. However, antibodies HP-1 and HP-3 showed cross-reactivity with rabbit and rat platelet secretory phospholipases A2, which share common enzymatic and structural features with the human synovial enzyme. Antibodies HP-1, HP-2 and HP-3 inhibited the activity of human synovial phospholipase A2. The antibodies were used to develop a rapid immunoaffinity column chromatographic procedure for enzyme purification. In some preparations, the recovery of total activity after immunoaffinity column chromatography was more than 100% suggesting the existence of endogenous inhibitory factors of phospholipase A2 in human synovial fluid.
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