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  • Title: [Effect of Fasudil on miroglia and astrocytes in experimental autoimmune encephalomyelitis mice].
    Author: Li YH, Liu CY, Zhang PJ, Yu JZ, Ji N, Yan YY, Feng L, Zhang HF, Xiao BG, Ma CG.
    Journal: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi; 2012 Dec; 28(12):1242-5. PubMed ID: 23232512.
    Abstract:
    AIM: To explore the therapeutic effect of Fasudil and its possible mechanisms in experimental autoimmune encephalomyelitis (EAE) mice, mainly focusing on the roles of microglia and astrocytes in the treatment. METHODS: Female adult C57BL/6 mice were immunized with MOG35-55 to induce chronic EAE. Fasudil was injected on day 3 p.i. (early Fasudil treatment), or at the onset of EAE (late Fasudil treatment). Normal saline was injected in other mice as EAE controls in a similar manner. Clinical score and body mass were recorded every other day. The expressions of iNOS on microglia and p-NF-κB/p65 on astrocytes were measured by immunohistochemistry and Western blotting. The levels of IL-1β and TNF-α in spinal cord homogenate were determined by ELISA. RESULTS: Fasudil delayed onset and ameliorated the severity of EAE. Fasudil inhibited the expression of iNOS on microglia and p-NF-κB/p65 on astrocytes in spinal cords, accompanied by the inhibition of inflammatory factors IL-1β and TNF-α. CONCLUSION: Fasudil exhibits therapeutic effect on EAE, possibly through inhibiting inflammatory molecules on microglia and astrocyte.
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