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  • Title: [Inhibitory effect of arsenic trioxide on invasion in human hepatocellular carcinoma SMMC-7721 cells and its mechanism].
    Author: Li HY, Cao LM.
    Journal: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi; 2012 Dec; 28(12):1254-7. PubMed ID: 23232515.
    Abstract:
    AIM: To investigate the effects of arsenic trioxide (As(2);O(3);) on human hepatocellular carcinoma SMMC-7721 cells' migration and invasion and the underlying molecular mechanism. METHODS: The effects of As(2);O(3); on cell migration and invasion were examined by wound-healing and Transwell assays; The expressions of CD147 and MMP-2 at both mRNA and protein levels in SMMC-7721 cells treated with As(2);O(3); were determined by real-time PCR and Western blotting, respectively. RESULTS: The wound-healing assay showed that the scuffing distance of SMMC-7721 cells exposed to 2.0 μmol/L As(2);O(3); for 12 and 24 h was significantly wider as compared with the ones unexposed to As(2);O(3);, indicating As(2);O(3); could inhibit the migration in SMMC-7721 cells (P<0.05). The Transwel1 assay showed that the number of SMMC-7721 cells migrating through the Matrigel treated by 2.0 μmol/L As(2);O(3); for 12 and 24 h obviously decreased as compared with non-treated ones, indicating that As(2);O(3); could inhibit the invasion of SMMC-7721 cells (P<0.05). Real-time PCR showed that the expressions of CD147 and MMP-2 mRNA were down-regulated in SMMC-7721 cells treated by 2.0 μmol/L As(2);O(3); for 6, 12 and 24 h, respectively (P<0.05). Western blotting demonstrated that the expressions of CD147 and MMP-2 proteins decreased in SMMC-7721 cells exposed to 2.0 μmol/L As(2);O(3); for 12, 24 and 48 h, respectively (P<0.05). CONCLUSION: As(2);O(3); could inhibit the migration and invasion in human hepatocellular carcinoma SMMC-7721 cells, which may be related to the down-regulation of CD147 and MMP-2.
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