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  • Title: [Cloning and expression of luxS and pfs and in vitro biosynthesis autoinducer 2 of avian pathogenic Escherichia coli from Anhui Province].
    Author: Han X, Bai H, Liu L, Chen W, Ding C, Hu Q, Qi K, Yu S.
    Journal: Wei Sheng Wu Xue Bao; 2012 Sep 04; 52(9):1167-72. PubMed ID: 23236852.
    Abstract:
    OBJECTIVE: The LuxS/AI-2 quorum sensing (QS) system shared by Gram-positive and Gram-negative bacteria involves the production of autoinducer-2 (AI-2). In this study, the method of biosynthesis of AI-2 was established using recombinant LuxS and Pfs of avian pathogenic Escherichia coli (APEC), which will be benefit for future study of the role of AI-2 in APEC. METHODS: We investigated AI-2 production in APEC by vibrio harveyi BB170 (BB170). Furthermore, APEC LuxS and Pfs were expressed, purified and used to investigate the production of AI-2 in vitro. LuxS and Pfs were incubated with S-ribosylhomocysteine (SAH), the reaction was detected for the production of luminescence of BB170. RESULTS: APEC can produce AI-2 by BB170 bioassay. Purified LuxS and Pfs enzymes incubated with SAH and produced 300 micromol/L AI-2 in the reaction products. CONCLUSION: The results demonstrated that recombinant Pfs and LuxS synthesize AI-2 in vitro from SAH. These findings will be of benefit to future studies of the role of AI-2 in APEC.
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