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  • Title: Isolation of cytopathogenic Rotavirus from neonatal calves.
    Author: Köves B.
    Journal: Acta Microbiol Acad Sci Hung; 1979; 26(3):225-31. PubMed ID: 232369.
    Abstract:
    Rotavirus was isolated in rolled calf kidney cultures from the intestinal contents of a calf suffering from diarrhoea. The cytopathic effect was demonstrated in native cultures as well as in stained preparations. The affected cells were elongated, became sickle-like in shape, disintegrated and detached from the monolayer. Virus-specific direct immunofluorescence ran parallel, in both time and intensity, with the cytopathic effects. Immuno-electron microscopy showed aggregated virus particles corresponding in size, shape and structure to Rotavirus virion.
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