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  • Title: Extracellular calcium ions are required for muscarine-sensitive potassium current in bullfrog sympathetic neurons.
    Author: Tokimasa T, Akasu T.
    Journal: J Auton Nerv Syst; 1990 Feb; 29(2):163-74. PubMed ID: 2324427.
    Abstract:
    Cultured bullfrog sympathetic neurons were voltage-clamped in the whole-cell configuration. The extracellular medium contained tetrodotoxin (3 microM) and cesium (1 mM) to block and inward sodium current and a hyperpolarization-activated cation current Attempts were made to separate the M-current from four other potassium currents. Tetraethylammonium (30 mM) was used to block a classical delayed rectifier current (IK) and a fast calcium-activated current (IC). Apamin (30 nM) was used to block a slow calcium-activated current (IAHP). 4-Aminopyridine (1 mM) was used to reduce the amplitude of a transient current (IA). In these conditions, the maximum M-conductance near 0 mV was reduced by as much as 90% when divalent cations such as cobalt (1 mM) were added to the superfusate. The maximum M-conductance was also reduced by as much as 60% when calcium ions were removed from the superfusate. The half-activation voltage in the steady-state activation curve and the reversal potential of the M-current were not significantly changed in the calcium-free solution. It is suggested that the presence of calcium ions in the extracellular space is required for the M-current activation.
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