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  • Title: Identification of a fatty acid ∆(6)-desaturase gene from the eicosapentaenoic acid-producing fungus Pythium splendens RBB-5.
    Author: Zhang R, Zhu Y, Ren L, Zhou P, Hu J, Yu L.
    Journal: Biotechnol Lett; 2013 Mar; 35(3):431-8. PubMed ID: 23247564.
    Abstract:
    A new full-length cDNA (PsD6) putatively encoding a ∆(6)-desaturase was cloned from the eicosapentaenoic acid-producing fungus Pythium splendens RBB-5. PsD6 contained an open reading frame of 1380 bp encoding a protein of 459 amino acids. The deduced amino acid sequence showed high similarity to those of other ∆(6)-desaturases. A recombinant vector expressing PsD6 (pPIC3.5K-D6) was constructed and transformed into Pichia pastoris GS115. The heterologous expressed PsD6 in P. pastoris desaturated linoleic acid to γ-linolenic acid but not desaturated α-linolenic acid to stearidonic acid. The results indicated that PsD6 was a fatty acid ∆(6)-desaturase and it had a substrate specificity for linoleic acid.
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