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  • Title: [Kinetic parameters of Na,K-ATPase in the gills of crabs adapted to freshened sea water].
    Author: Busev VM, Semen'kov PG.
    Journal: Biokhimiia; 1979 Nov; 44(11):2048-52. PubMed ID: 232667.
    Abstract:
    The dependence of Na,K-ATPase activity on concentrations of ATP, Na+, K+, Mg2+ and ouabain in the membrane preparations of crab gills was studied. The first group of crabs was adapted to freshened (25%) and the second one--to normal (100%) sea water. A 40-day adaptation of crabs to the freshened sea water results in an increase of maximal activity of Na,K-ATPase, but does not affect the enzyme affinity for ATP, Na+, K+, Mg2+ and ouabain, as well as its cooperative properties. It is assumed that adaptation of crabs to freshened sea water is accompanied by an accumulation of Na, K-ATPase in the epithelial cell membranes or crab gills without causing any qualitative changes of the enzyme.
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