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  • Title: Bovine monoclonal antibodies specific for bovine herpesvirus-1 glycoprotein gIII.
    Author: Srikumaran S, Onisk DV, Zamb TJ, Osorio FA, Rice DN.
    Journal: Am J Vet Res; 1990 Apr; 51(4):543-5. PubMed ID: 2327611.
    Abstract:
    Spleen cells from a calf immunized with bovine herpesvirus-1 (BHV-1) were fused with the nonsecreting murine cell line SP2/0. Several bovine-murine hybridomas secreting bovine immunoglobulins were stabilized. Of these, 9 hybridomas secreted bovine monoclonal antibodies that specifically bound to BHV-1 in a radioimmunoassay. Two of these monoclonal antibodies reacted specifically with BHV-1 in an indirect fluorescent antibody test and immunoprecipitated a BHV-1 glycoprotein with molecular mass of 97 kilodaltons.
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