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  • Title: Isolation and structural analysis of three new disialylated oligosaccharides from human milk.
    Author: Grönberg G, Lipniunas P, Lundgren T, Lindh F, Nilsson B.
    Journal: Arch Biochem Biophys; 1990 May 01; 278(2):297-311. PubMed ID: 2327786.
    Abstract:
    Five disialylated oligosaccharides, of which three were new compounds, were isolated from pooled human milk. The isolation procedure included gel filtration and ion-exchange chromatography and the final purification was accomplished by HPLC using a mobile phase containing triethylamine as an ion-pairing reagent. Structural analysis was carried out by chemical analyses, fast atom bombardment mass spectrometry, and 500-MHz NMR spectroscopy. The following new structures were found: (formula; see text).
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