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  • Title: Na+/K+-dependent adenosinetriphosphatase in rabbit kidney. Separation by affinity chromatography.
    Author: Giunta C, De Bortoli M, Brigidi R.
    Journal: Boll Soc Ital Biol Sper; 1979 Sep 15; 55(17):1659-65. PubMed ID: 233068.
    Abstract:
    We attempted separations by affinity chromatography of the Na+/K+-dependent adenosinetriphosphatase, using Sepharose 6B covalently coupled with ouabain, either on microsomal fractions or on preparations purified by discontinuous sucrose density gradient ultracentrifugation. The material, specifically eluted with ouabain, was tested to evaluate the recovery of ATPase activity and ouabain binding capacity. The results have shown the efficiency of this technique for (Na+/K+)-APTase isolation.
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