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  • Title: Discrimination of isobaric Leu/Ile residues by MALDI in-source decay mass spectrometry.
    Author: Asakawa D, Smargiasso N, De Pauw E.
    Journal: J Am Soc Mass Spectrom; 2013 Feb; 24(2):297-300. PubMed ID: 23307320.
    Abstract:
    MALDI in-source decay (ISD) has been used for top-down sequencing of proteins. The use of the matrix 1,5-diaminonapthalene (1,5-DAN) gave abundant w ions, which are formed from the unimolecular dissociation of z• radical fragments via α cleavage reaction and thus help identify which of the isobaric amino acids, Leu or Ile, is present. The high abundance of w ions in MALDI-ISD with 1,5-DAN results from the low collision rate in the MALDI plume. MALDI-ISD with 1,5-DAN appears to be an useful method for the top-down sequencing of proteins, including discrimination of Leu and Ile near the C-terminal end.
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