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  • Title: Separation and fractionation of exopolysaccharides from Porphyridium cruentum.
    Author: Patel AK, Laroche C, Marcati A, Ursu AV, Jubeau S, Marchal L, Petit E, Djelveh G, Michaud P.
    Journal: Bioresour Technol; 2013 Oct; 145():345-50. PubMed ID: 23313179.
    Abstract:
    In this work the extraction of EPSs from culture media of Porphyridium cruentum, by dialysis, solvent-precipitation with 3 polar alcohols (methanol, ethanol and isopropanol) and membrane separation techniques has been studied. Diafiltration (DF) using a membrane with a 300 kDa molecular weight cut off was the most efficient technique compared to solvent-extraction and dialysis methods. After extraction, EPS fraction was characterized in terms of rheological properties and biochemical content. The product exhibited shear thinning behavior and a critical overlap concentration equal to 0.6 g/L. The monosaccharide composition was investigated after acidic hydrolysis. Xylose, galactose, glucose and glucuronic acid were identified as the main constitutive monomers.
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