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  • Title: Isolation and characterization of antibodies to Clostridium perfringens epsilon toxin from hyperimmune horse serum.
    Author: Worthington RW, Mülders MS.
    Journal: Onderstepoort J Vet Res; 1979 Sep; 46(3):121-4. PubMed ID: 233148.
    Abstract:
    Antibodies against epsilon toxin were isolated from hyperimmune horse serum by affinity chromatography. Purified epsilon prototoxin covalently bound to Affigel 202 was used as immunosorbent, and antibodies were eluted with 6.0 M guanidine chloride. In a single run 80 mg of antibody could be recovered from a 20 microliter column of immunosorbent. The antibody was shown to belong to the IgG(T) class of immunoglobulins.
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