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Title: Hoeflea halophila sp. nov., a novel bacterium isolated from marine sediment of the East Sea, Korea. Author: Jung MY, Shin KS, Kim S, Kim SJ, Park SJ, Kim JG, Cha IT, Kim MN, Rhee SK. Journal: Antonie Van Leeuwenhoek; 2013 May; 103(5):971-8. PubMed ID: 23314912. Abstract: A Gram-negative, aerobic, motile, straight or curved rod-shaped marine bacterium was isolated from marine sediment of the East Sea, Korea. The isolated strain, JG120-1(T), grows with 0-5 % (w/v) NaCl and at 15-30 °C and pH 6-9. α-galactosidase activity test was positive. Comparative 16S rRNA gene sequence studies showed that this strain belonged to the Alphaproteobacteria and was the most closely related to Hoeflea alexandrii AM1 V30(T), Hoeflea phototrophica DFL-43(T) and Hoeflea marina LMG 128(T) (98.9, 97.9 and 97.0 % 16S rRNA gene sequence similarities, respectively). Strain JG120-1(T) was found to possess summed feature 8 (C18:1ω7c/C18:1ω6c, 71.11 %) as the major cellular fatty acid. The major ubiquinone was determined to be Q-10. Polar lipids include phosphatidylglycerol, phosphatidylethanolamine, sulfoquinovosyl diacylglycerol, phosphatidylcholine and phosphatidylmonomethylethanolamine. The G+C content of the genomic DNA of strain JG120-1(T) was determined to be 57.8 mol %. DNA-DNA relatedness data indicated that strain JG120-1(T) represents a distinct species that is separate from H. phototrophica DFL-43(T), H. marina LMG128(T) and H. alexandrii AM1 V30(T). On the basis of polyphasic evidences, it is proposed that strain JG120-1(T) (= KCTC 23107(T) = JCM 16715(T)) represents the type strain of a novel species, Hoeflea halophila sp. nov.[Abstract] [Full Text] [Related] [New Search]