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  • Title: Phosphorylation of ornithine decarboxylase in intact erythroleukemia cells.
    Author: Flamigni F, Marmiroli S, Meggio F, Guarnieri C, Pinna LA.
    Journal: Biochim Biophys Acta; 1990 May 02; 1052(2):345-7. PubMed ID: 2334742.
    Abstract:
    32P-labeled ornithine decarboxylase was isolated by immunoprecipitation from murine erythroleukemia cells incubated in a medium containing [32P]ortophosphoric acid. Analysis of immunoprecipitate by SDS-polyacrylamide gel electrophoresis and autoradiography revealed a radiolabeled band, which corresponded to the position of mouse ornithine decarboxylase, phosphorylated in vitro by casein kinase-2. A preparation of casein kinase-2 purified from nuclei of erythroleukemia cells could also phosphorylate mouse ornithine decarboxylase.
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