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  • Title: Method to convert N-terminal glutamine to pyroglutamate for characterization of recombinant monoclonal antibodies.
    Author: Xu W, Peng Y, Wang F, Paporello B, Richardson D, Liu H.
    Journal: Anal Biochem; 2013 May 01; 436(1):10-2. PubMed ID: 23357233.
    Abstract:
    Cyclization of N-terminal glutamine to pyroglutamate is a common modification of recombinant monoclonal antibodies that has often been identified by liquid chromatography mass spectrometry (LC-MS) analysis using separated fractions. An alternative approach of using glutaminyl-peptide cyclotransferase to convert the N-terminal glutamine to pyroglutamate was developed in the current study. Enzymatic conversion of the N-terminal glutamine to pyroglutamate not only provides an identification of the N-terminal amino acids without fraction collection but also can significantly simplify the chromatograms to assist fraction collections for the characterization of other antibody variants.
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