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  • Title: Analysis of resistant starches in rat cecal contents using Fourier transform infrared photoacoustic spectroscopy.
    Author: Anderson TJ, Ai Y, Jones RW, Houk RS, Jane JL, Zhao Y, Birt DF, McClelland JF.
    Journal: J Agric Food Chem; 2013 Feb 27; 61(8):1818-22. PubMed ID: 23360415.
    Abstract:
    Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) qualitatively and quantitatively measured resistant starch (RS) in rat cecal contents. Fisher 344 rats were fed diets of 55% (w/w, dry basis) starch for 8 weeks. Cecal contents were collected from sacrificed rats. A corn starch control was compared against three RS diets. The RS diets were high-amylose corn starch (HA7), HA7 chemically modified with octenyl succinic anhydride, and stearic-acid-complexed HA7 starch. To calibrate the FTIR-PAS analysis, samples from each diet were analyzed using an enzymatic assay. A partial least-squares cross-validation plot generated from the enzymatic assay and FTIR-PAS spectral results for starch fit the ideal curve with a R(2) of 0.997. A principal component analysis plot of components 1 and 2 showed that spectra from diets clustered significantly from each other. This study clearly showed that FTIR-PAS can accurately quantify starch content and identify the form of starch in complex matrices.
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