These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Fluorescent detection of lead in environmental water and urine samples using enzyme mimics of catechin-synthesized Au nanoparticles.
    Author: Wu YS, Huang FF, Lin YW.
    Journal: ACS Appl Mater Interfaces; 2013 Feb; 5(4):1503-9. PubMed ID: 23369297.
    Abstract:
    A facile, cost-effective, and sensitive fluorescent method for Pb²⁺ ion detection had been developed using catechin synthesized gold nanoparticles (C-Au NPs). The Pb-catechin complexes and Pb-Au alloys that formed on the C-Au NPs surfaces allowed NPs to exhibit peroxidase-mimicking catalytic activity in the H₂O₂-mediated oxidation of Amplex UltraRed (AUR). In 5 mM Tris-acetate buffers at pH 7.0, the H₂O₂-AUR-C-Au NP probe was highly selective (>100-fold) for Pb²⁺ ions in the presence of other tested metal ions (K⁺, Ag⁺, Na⁺, Cd²⁺, Ni²⁺, Ca²⁺, Hg²⁺, Sr²⁺, Co²⁺, Cu²⁺, Ba²⁺, Fe²⁺, Mg²⁺, Cr³⁺, and Fe³⁺ ions). The fluorescence intensity (excitation/emission maxima ∼540/588 nm) of the AUR product was proportional to the concentration of Pb²⁺ ions in the range of 10 nM-1.0 μM with a linear correlation (R² = 0.99). The H₂O₂-AUR-C-Au NP probe detected Pb²⁺ ions with a limit of detection (signal-to-noise ratio: 3) of 1.5 nM. The practicality of the H₂O₂-AUR-C-Au NP probe was validated for the determination of Pb²⁺ ion concentration in environmental water and urine samples, demonstrating its advantages of simplicity, selectivity, and sensitivity.
    [Abstract] [Full Text] [Related] [New Search]