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  • Title: Polyphenolics composition of the leaves of Zanthoxylum bungeanum Maxim. grown in Hebei, China, and their radical scavenging activities.
    Author: Yang LC, Li R, Tan J, Jiang ZT.
    Journal: J Agric Food Chem; 2013 Feb 27; 61(8):1772-8. PubMed ID: 23383696.
    Abstract:
    High performance liquid chromatography coupled with tandem mass spectrometry with electrospray ionization and negative ion detection (HPLC-ESI-MS/MS) was used to identify the polyphenols from the leaves of a traditional vegetable and spice, Zanthoxylum bungeanum Maxim., in China by comparison of the retention times and mass spectral fragment with standard substances or related literature. A phenolic acid glucoside, two acids, and eight flavonoids were determined including vanillic acid-4-glucoside (22.75 μg/g, dry weight of leaves), quinic acid (58.58 μg/g), chlorogenic acid (2515.96 μg/g), epicatechin (77.80 μg/g), 5-feruloyquinic acid (16.63 μg/g), syringetin-3-glucoside (103.23 μg/g), rutin (89.41 μg/g), hyperoside (886.36 μg/g), quercetin-3-arabinoside (118.75 μg/g), quercitrin (645.82 μg/g), and isorhamnetin-3-glucoside (104.27 μg/g). The obtained polyphenols show good radical scavenging activities in DPPH and superoxide anion. Through the intracellular antioxidation assay, the result shows that the polyphenols could reduce the ROS in HT-29 cells without cell toxicity.
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