These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Preparation of D-gulose from disaccharide lactitol using microbial and chemical methods.
    Author: Morimoto K, Shimonishi T, Miyake S, Takata G, Izumori K.
    Journal: Biosci Biotechnol Biochem; 2013; 77(2):253-8. PubMed ID: 23391912.
    Abstract:
    When an M31 strain of Agrobacterium tumefaciens was grown in a mineral salt medium at 30 °C containing 1.0% lactitol as sole carbon source, a keto-sugar was efficiently accumulated in the supernatant. This oxidation from lactitol to the keto-sugar was caused by M31 cells grown with medium containing a disaccharide unit, including sucrose, lactitol, lactose, maltose, or maltitol, suggesting that the enzyme is inducible. M31 also demonstrated good growth characteristics in Tryptic Soy Broth (TSB) medium containing 1.0% sucrose, and cells grown under these conditions showed strong lactitol transformation activity. The keto-sugar product was reduced by chemical hydrogenation and the resulting product was hydrolyzed to D-gulose, D-galactose, and D-sorbitol by acid hydrolysis, revealing that the reduced products are lactitol and D-gulosyl-(β-1,4)-D-sorbitol. Taken together, these results indicate that M31 can convert lactitol to 3-ketolactitol and thus provide access to the rare sugar D-gulose.
    [Abstract] [Full Text] [Related] [New Search]