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  • Title: Development and validation of a rapid ultra high pressure liquid chromatographic method for the determination of methylxanthines in herbal infusions.
    Author: Zacharis CK, Kika FS, Tzanavaras PD, Fytianos K.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2013 May 15; 927():218-22. PubMed ID: 23394897.
    Abstract:
    An ultra high pressure liquid chromatographic method coupled with diode array detector (UHPLC-DAD) has been developed and validated for the fast separation and determination of three major methylxanthines, i.e., caffeine, theophylline and theobromine, in various herbal beverages. Isocratic elution using 0.1vol% formic acid/CH3OH (92.5:7.5, v/v) enabled the completion of the separation cycle in less than 3min using a flow rate of 0.7mL/min and a column temperature of 50°C. Validation of the method included linearity (0.5-50mg/L), limits of detection (12-35μg/L) and quantification (40-120μg/L), precision, matrix effect and accuracy. The percent recoveries ranged between 90 and 108%.
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