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  • Title: A single amino acid determines position specificity of an Arabidopsis thaliana CCoAOMT-like O-methyltransferase.
    Author: Wils CR, Brandt W, Manke K, Vogt T.
    Journal: FEBS Lett; 2013 Mar 18; 587(6):683-9. PubMed ID: 23416302.
    Abstract:
    Caffeoyl-coenzyme A O-methyltransferase (CCoAOMT)-like proteins from plants display a conserved position specificity towards the meta-position of aromatic vicinal dihydroxy groups, consistent with the methylation pattern observed in vivo. A CCoAOMT-like enzyme identified from Arabidopsis thaliana encoded by the gene At4g26220 shows a strong preference for methylating the para position of flavanones and dihydroflavonols, whereas flavones and flavonols are methylated in the meta-position. Sequence alignments and homology modelling identified several unique amino acids compared to motifs of other CCoAOMT-like enzymes. Mutation of a single glycine, G46 towards a tyrosine was sufficient for a reversal of the unusual para- back to meta-O-methylation of flavanones and dihydroflavonols.
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