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  • Title: [Impact of high-fat diet induced obesity on glucose absorption in small intestinal mucose in rats].
    Author: Huang W, Liu R, Guo W, Wei N, Qiang O, Li X, Ou Y, Tang C.
    Journal: Wei Sheng Yan Jiu; 2012 Nov; 41(6):878-82. PubMed ID: 23424860.
    Abstract:
    OBJECTIVE: To investigate whether high-fat diet induced obesity was associated with variation of glucose absorption in small intestinal mucosa of rats. METHODS: 46 male SD rats were randomly divided into high-fat diet group (n = 31) and control group (n = 15), fed with high-fat diet and normal diet for 24 weeks, respectively. After 24 weeks, the rats were divided into obese (n = 16) and obesity-resistant group (n = 10) according to their body weight. Rats' body weight, abdominal fat weight, plasma glucose level, maltase, sucrase activity in small intestinal mucosa were measured. SGLT-1 expression in intestinal mucosa was detected by immunohistochemistry, RT-PCR and Western blot. RESULTS: Mean body weight, abdominal fat weight, fast plasma glucose levels, maltase activities and SGLT-1 protein expression in intestinal mucosa of obese rats were significantly higher than those in the control and obesity-resistant rats (P < 0.05). Sucrase activities in intestinal mucosa showed no statistical difference among the three groups (P > 0.05). The SGLT-1 mRNA expression in obese group was increased by 12.5% and 23% when compare with the control and obesity-resistant group, respectively. But the difference was not statistical significant (P > 0.05). CONCLUSION: High-fat diet induced obesity was associated with the increased intestinal maltase activity and expression of SGLT-1 in rats, the key molecule in glucose absorption.
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