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  • Title: [Use of Amplicor HIV-1 DNA test v.1.5 in diagnosis of perinatal infection from HIV-1 in Cuba].
    Author: Machado Zaldivar LY, Blanco de Armas M, Lubián Caballero AL, Díaz Torres HM.
    Journal: Rev Cubana Med Trop; 2010; 62(2):154-6. PubMed ID: 23431628.
    Abstract:
    INTRODUCTION: HIV-1 proviral DNA amplification is the preferential method for HIV diagnosis infection in infants and it has been used in Cuba since 1992. AMPLICOR HIV-1 DNA kit is an in vitro qualitative assay for the detection of HIV-1 proviral DNA in the whole blood. OBJECTIVE: this paper showed the results of the use of this kit for the first time in Cuba for the perinatal diagnosis of HIV-1 infection. METHODS: three hundred forty six whole blood samples from children of HIV seropositive women were analyzed by the AMPLICOR HIV-1 DNA kit in the period 2005-2007. RESULTS: among the tested samples, six were positive, and 340 negative. CONCLUSIONS: the assay was reproducible under the Cuban conditions and the achieved results made the diagnosis and follow up of children of HIV-1 seropositive mothers possible.
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