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  • Title: [Selective interaction of antipeptide antibodies to N-terminal site of lactate dehydrogenase-5 from swine muscle with various isoforms of human lactate dehydrogenase].
    Author: Alekseeva AE, Potapkina TA, Prozorovskiĭ VN.
    Journal: Vopr Med Khim; 1990; 36(1):73-5. PubMed ID: 2343581.
    Abstract:
    Antipeptide antibodies produced towards N-terminal site of lactate dehydrogenase-5 (LDH5) from pig muscular tissue exhibited specific reactions with N-terminal site of pig LDH5 and the whole enzyme molecule. An immunosorbent containing the antipeptide antibodies produced bound and precipitated all the LDH isoforms involving even a single M-subunit of the enzyme (LDH2, LDH5) but did not precipitate H4-isoform (LDH1) in extracts of human heart and skeletal muscle tissues and in blood serum of patients with myocardium infarction. The data obtained suggest that antipeptide antibodies against N-terminal site of pig muscular tissue LDH reacted selectively only with M-subunits of human LDH and did not interact with H4 isoform, which occurred due to dissimilarities in primary structure of N-terminal sites of M4 and H4 LDH isoenzymes.
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