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  • Title: Orthogonal spin labeling and Gd(III)-nitroxide distance measurements on bacteriophage T4-lysozyme.
    Author: Garbuio L, Bordignon E, Brooks EK, Hubbell WL, Jeschke G, Yulikov M.
    Journal: J Phys Chem B; 2013 Mar 21; 117(11):3145-53. PubMed ID: 23442004.
    Abstract:
    We present the first example of chemoselective site-specific spin labeling of a monomeric protein with two spectroscopically orthogonal spin labels: a gadolinium(III) chelate complex and a nitroxide radical. A detailed analysis of the performance of two commercially available Gd(III) ligands in the Gd(III)-nitroxide pulse double electron-electron resonance (DEER or PELDOR) experiment is reported. A modification of the flip angle of the pump pulse in the Gd(III)-nitroxide DEER experiment is proposed to optimize sensitivity.
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