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  • Title: Bead-based padlock rolling circle amplification for single DNA molecule counting.
    Author: Sato K, Ishii R, Sasaki N, Sato K, Nilsson M.
    Journal: Anal Biochem; 2013 Jun 01; 437(1):43-5. PubMed ID: 23467098.
    Abstract:
    Padlock rolling circle amplification (RCA) is a powerful analytical method for ultrasensitive DNA detection. Although there are some advantages to bead-based RCA, a detailed study of the relationship between the bead material and the efficiency of bead-based RCA has not been reported. Here, we compared the reaction efficiencies of bead-based RCA performed on two types of bead material: agarose and polystyrene. Agarose was a more suitable material for on-bead RCA. The calibration curve showed linearity between 0.05 and 1 nM, and the limit of detection was 9 pM (9 amol) for Salmonella DNA determination.
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